PRP, or platelet-rich plasma, what is it exactly? An Overview of PRP is given here.
What is PRP?
It is a state-of-the-art cutting-edge healing technique, which we have the ability and to jump-start the healing process, enhanced and speed up the healing process to help fasten the recovery process after injury or after a chronic inflammatory process such as the osteoarthritis of the knee joint pain. This is a real magic how mankind is able to interfere in the normal physiological process of healing. In so doing we can shorten the process to a lot more faster. It heals by stopping any further damage as well as repair the damages done to fully recover. Both the 2 processes are going on side by side.
The whole-blood after the separation by spinning is separated into 3 fractions. the lower red portion is the red blood cell (RBC) is discarded as it do not helps in the healing. the top portion is the platelet-poor plasma, PPP which do not has sufficient growth factors or “tonic” for the healing is discarded. the small fraction sandwiched between these 2 layers is the PRP which has a high concentration of good “tonic” factors for the healing.
The PRP uses an autologous, meaning one’s own blood cell, called the platelet for healing and repair. Furthermore, the growth factors present in the plasma participates to help in improving the scar, acne scaring, and skin tone and texture, and even for hair re-grow. This is not to mention it has the healing property for the injured tissue.
The collected platelet cell can be activated by addition of Calcium chloride or Calcium gluconate or thrombin so that the bio-active protein can be released from the alpha granules, a process called degranulation.
For every 10 ml of whole blood, there is less than 1 ml of PRP together with the growth factors and cytokines. It is therefore a highly concentrated collection of platelet and growth factors. The fraction is then injected locally directly onto and into the injured tissue (the recipient site) to initiate and jump-start the repair and healing process instantly.
So in the aesthetic medicine use, it help one to look better; and in the use for professional athlete’s injury it helps to fasten the recovery. The recovery is so fast and is even believed to enhance the athlete’s performance that its use was banned at one point in time but not anymore. It speeds up muscle injury repair. At our clinic, we the doctors do for both applications. This procedure has been used by the market for more than 20 years.
PRP for Aesthetics use
PRP for facial aesthetic and rejuvenation is called Vampire Facelift. It has been used since 2006. The treatment is good for the improvement of skin color tone as well as the texture. The PRP is rich in various Platelet Derived Growth Factors, PDGFs in short.
These growth factors, PDGF promote regeneration of fibroblast and keratinocyte and increased production of collagen and keratin respectively. PDGF also help in cellular division of fibroblast. The fibroblast is present in abundant in the connective tissues. Fibroblast also produce the extracellular matrix other than collagen. The production of both cellular matrix and collagen is the very reason that it help to decrease the wrinkle for aesthetic purpose.
This procedure is very conservative and minimally invasive. The facelift effect is not comparable to the traditional classical facelift surgery, but its effect is sufficient to bring about improvement in skin tone, texture and color. The result can be seen within 3 weeks and improvement continue for the next 6 to 12 months as more collagen is being laid within the skin matrix. The result may last 1 to 2 years. The area might be treated include the Crow’s feet, the cheeks peri-oral line, naso-labial or power line, jawline, neck and chest. The procedure can take 1 to 2 hours depending on the extend and complexity. For best result, repeat in 2 to four weeks. More the treatment, better is the result.
Content of PRP
|Content of PRP
The whole blood after spinning, we get RBC at the bottom due to its higher density, PPP at the top because it has a lighter density, PRP at the plasma and RBC interface, as its density is in the middle of the other two.
|PRP contain many growth factors & cytokines as below, although this list is in no way exhaustive:
· Transforming growth factor beta (TGFβ) – for cell division & attract stem cell to the wound
· Connective tissue growth factor (for wound healing)
· Insulin-like growth factor I (IGF-1)
· Vascular-endothelial growth factor (for organ regeneration)
· EGF, Epithelial growth factor
· Interleukin 8
· Fibroblast growth factor ( for wound healing/ aesthetics uses)
· Keratinocyte growth factor (wound healing/ aesthetics uses)
Wound healing process
|What is PRP and how does it help in wound healing? It is very much dependent on this line of reasoning. Once the PRP proteins, growth factor, cytokines bind to cellular receptors of skin such as in aesthetics applications, tendon, ligament, fascia, muscle, and bone and cartilages, lower back structures such as application in orthopedic, as in soft tissue injury, usually in such cell as fibroblast, endothelial cell and osteoblast, they activate inter-cellular events helping to activate the following processes of wound healing in this particular order as stated below:|
|· Angiogenesis (this is the proliferation of new blood vessels, like the artery and the vein, as well as the lymphatic)
· Migration of stem cell to the injection area, or to the injury site
· New cell proliferation ensues at the injury site
· And finally the production of extra-cellular matrix at the injury site
|The first results start to be visible from about 3 weeks. The effect can easily last up to 18 months or more.|